rv b14 Search Results


rv b14 vrmc 7 infectious clones  (ATCC)
92
ATCC rv b14 vrmc 7 infectious clones
Rv B14 Vrmc 7 Infectious Clones, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rv+b14/pWR3%2E26+purified+plasmid+DNA/pm42108281-158-3-10
Average 92 stars, based on 1 article reviews
rv b14 vrmc 7 infectious clones - by Bioz Stars, 2026-10
92/100 stars
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rv b14 replicon rna  (New England Biolabs)
97
New England Biolabs rv b14 replicon rna
a, <t>RV-B14</t> <t>replicon</t> or firefly luciferase mRNA control luciferase expression in wild-type (WT; H1-HeLa +CDHR3 ), SETD3 knockout (SETD3 KO ), and SETD3 stably complemented (SETD3 KO + SETD3 cDNA) cell lines. Top panel is a schematic for RV-B14 replicon construct, in which Firefly luciferase reporter gene (FLuc) was inserted to replace the majority of the structural proteins b, Schematic for enterovirus infection cycle and inhibitors that block specific stages during viral infection are indicated. Synchronized luciferase-expressing CV-B3 infection of SETD3 KO cells and WT cells treated with either 125 μM cycloheximide (CHX) or 2 mM guanidine hydrochloride (GuHCl) over a time course (right panel). c, Confocal imaging of SETD3 KO cells and SETD3 KO cells that stably expressed GFP, SETD3 fused with GFP at the N-terminal (GFP-SETD3), GFP-SETD3 fused with a nuclear localization signal (GFP-SETD3-NLS), or GFP-SETD3 fused with a nuclear export signal (GFP-SETD3-NES). A representative set of two independent experiments is shown here. Scale bars, 7 μm. d, qRT-PCR quantification of viral <t>RNA</t> levels in RV-C15 or EV-D68 MO infected cell lines. All of the complemented constructs were expressed under a minimal expression promoter. For panel a , b , and d, datasets represent the mean with SEM of n = 3 independent biological replicates. P -values were determined by either two-way ANOVA ( a and b ) or ordinary one-way ANOVA ( d ) with Holm-Sidak correction on log-transformed data. For panel b , at 4 hpi and beyond, all differences observed between untreated WT vs. all other conditions are statistically significant ( P < 0.0001). ns = non-significant. * = P < 0.05. ** = P < 0.0001.
Rv B14 Replicon Rna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rv+b14/SmaI/pmc06879830-235-0-8
Average 97 stars, based on 1 article reviews
rv b14 replicon rna - by Bioz Stars, 2026-10
97/100 stars
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rhinovirus 14  (ATCC)
94
ATCC rhinovirus 14
a, <t>RV-B14</t> <t>replicon</t> or firefly luciferase mRNA control luciferase expression in wild-type (WT; H1-HeLa +CDHR3 ), SETD3 knockout (SETD3 KO ), and SETD3 stably complemented (SETD3 KO + SETD3 cDNA) cell lines. Top panel is a schematic for RV-B14 replicon construct, in which Firefly luciferase reporter gene (FLuc) was inserted to replace the majority of the structural proteins b, Schematic for enterovirus infection cycle and inhibitors that block specific stages during viral infection are indicated. Synchronized luciferase-expressing CV-B3 infection of SETD3 KO cells and WT cells treated with either 125 μM cycloheximide (CHX) or 2 mM guanidine hydrochloride (GuHCl) over a time course (right panel). c, Confocal imaging of SETD3 KO cells and SETD3 KO cells that stably expressed GFP, SETD3 fused with GFP at the N-terminal (GFP-SETD3), GFP-SETD3 fused with a nuclear localization signal (GFP-SETD3-NLS), or GFP-SETD3 fused with a nuclear export signal (GFP-SETD3-NES). A representative set of two independent experiments is shown here. Scale bars, 7 μm. d, qRT-PCR quantification of viral <t>RNA</t> levels in RV-C15 or EV-D68 MO infected cell lines. All of the complemented constructs were expressed under a minimal expression promoter. For panel a , b , and d, datasets represent the mean with SEM of n = 3 independent biological replicates. P -values were determined by either two-way ANOVA ( a and b ) or ordinary one-way ANOVA ( d ) with Holm-Sidak correction on log-transformed data. For panel b , at 4 hpi and beyond, all differences observed between untreated WT vs. all other conditions are statistically significant ( P < 0.0001). ns = non-significant. * = P < 0.05. ** = P < 0.0001.
Rhinovirus 14, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rv+b14/Human+rhinovirus+14/pmc06089459-347-2-16
Average 94 stars, based on 1 article reviews
rhinovirus 14 - by Bioz Stars, 2026-10
94/100 stars
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rhinoviruses  (ATCC)
97
ATCC rhinoviruses
a, <t>RV-B14</t> <t>replicon</t> or firefly luciferase mRNA control luciferase expression in wild-type (WT; H1-HeLa +CDHR3 ), SETD3 knockout (SETD3 KO ), and SETD3 stably complemented (SETD3 KO + SETD3 cDNA) cell lines. Top panel is a schematic for RV-B14 replicon construct, in which Firefly luciferase reporter gene (FLuc) was inserted to replace the majority of the structural proteins b, Schematic for enterovirus infection cycle and inhibitors that block specific stages during viral infection are indicated. Synchronized luciferase-expressing CV-B3 infection of SETD3 KO cells and WT cells treated with either 125 μM cycloheximide (CHX) or 2 mM guanidine hydrochloride (GuHCl) over a time course (right panel). c, Confocal imaging of SETD3 KO cells and SETD3 KO cells that stably expressed GFP, SETD3 fused with GFP at the N-terminal (GFP-SETD3), GFP-SETD3 fused with a nuclear localization signal (GFP-SETD3-NLS), or GFP-SETD3 fused with a nuclear export signal (GFP-SETD3-NES). A representative set of two independent experiments is shown here. Scale bars, 7 μm. d, qRT-PCR quantification of viral <t>RNA</t> levels in RV-C15 or EV-D68 MO infected cell lines. All of the complemented constructs were expressed under a minimal expression promoter. For panel a , b , and d, datasets represent the mean with SEM of n = 3 independent biological replicates. P -values were determined by either two-way ANOVA ( a and b ) or ordinary one-way ANOVA ( d ) with Holm-Sidak correction on log-transformed data. For panel b , at 4 hpi and beyond, all differences observed between untreated WT vs. all other conditions are statistically significant ( P < 0.0001). ns = non-significant. * = P < 0.05. ** = P < 0.0001.
Rhinoviruses, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rv+b14/A-10/pm33894205-48-11-21
Average 97 stars, based on 1 article reviews
rhinoviruses - by Bioz Stars, 2026-10
97/100 stars
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rv b  (ZeptoMetrix corporation)
94
ZeptoMetrix corporation rv b
a, <t>RV-B14</t> <t>replicon</t> or firefly luciferase mRNA control luciferase expression in wild-type (WT; H1-HeLa +CDHR3 ), SETD3 knockout (SETD3 KO ), and SETD3 stably complemented (SETD3 KO + SETD3 cDNA) cell lines. Top panel is a schematic for RV-B14 replicon construct, in which Firefly luciferase reporter gene (FLuc) was inserted to replace the majority of the structural proteins b, Schematic for enterovirus infection cycle and inhibitors that block specific stages during viral infection are indicated. Synchronized luciferase-expressing CV-B3 infection of SETD3 KO cells and WT cells treated with either 125 μM cycloheximide (CHX) or 2 mM guanidine hydrochloride (GuHCl) over a time course (right panel). c, Confocal imaging of SETD3 KO cells and SETD3 KO cells that stably expressed GFP, SETD3 fused with GFP at the N-terminal (GFP-SETD3), GFP-SETD3 fused with a nuclear localization signal (GFP-SETD3-NLS), or GFP-SETD3 fused with a nuclear export signal (GFP-SETD3-NES). A representative set of two independent experiments is shown here. Scale bars, 7 μm. d, qRT-PCR quantification of viral <t>RNA</t> levels in RV-C15 or EV-D68 MO infected cell lines. All of the complemented constructs were expressed under a minimal expression promoter. For panel a , b , and d, datasets represent the mean with SEM of n = 3 independent biological replicates. P -values were determined by either two-way ANOVA ( a and b ) or ordinary one-way ANOVA ( d ) with Holm-Sidak correction on log-transformed data. For panel b , at 4 hpi and beyond, all differences observed between untreated WT vs. all other conditions are statistically significant ( P < 0.0001). ns = non-significant. * = P < 0.05. ** = P < 0.0001.
Rv B, supplied by ZeptoMetrix corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rv+b14/Rhinovirus+B14+Culture+Fluid/bio_rxiv__2023__05__04__539429-55-12-19
Average 94 stars, based on 1 article reviews
rv b - by Bioz Stars, 2026-10
94/100 stars
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rcombinant anti-human ervk-6 antibody scfv fragment  (Creative BioMart)
N/A
Recombinant Mouse Antibody scFv Fragment shows specific binding activity against Human ERVK-6, expressed in E. coli.Antibody assay: Enzyme-linked Immunosorbent Assay; Western blot; Immunofluorescence; Functional StudyStore at -20°C. Open under aseptic conditions.http://www.creativebiolabs.net/Rcombinant-Anti-Human-ERVK-6-Antibody-scFv-Fragment-5033.htm
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rcombinant anti-human ervk-6 antibody  (Creative BioMart)
N/A
Recombinant Mouse Antibody recognizes and binds to Human ERVK-6, expressed in Chinese Hamster Ovary cells(CHO).Antibody assay: Enzyme-linked Immunosorbent Assay; Western blot; Immunoprecipitation; Functional StudyShort term: store at 4°C (over 6 months), long term: -20°C or
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rcombinant anti-human ervk-6 antibody fab fragment  (Creative BioMart)
N/A
Recombinant Mouse Antibody Fab Fragment exhibits selective binding affinity to Human ERVK-6, expressed in Chinese Hamster Ovary cells(CHO).Antibody assay: Immunofluorescence; Western blot; Functional StudyShort term: store at 4°C (over 6 months), long term: -20°C or
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ervwe1 antibody  (biorbyt)
N/A
Rabbit Polyclonal to ERVWE1. Isotype Note: IgG Host Note: Rabbit Conjugation Note: Unconjugated Reactivity Note: Human Application Note: ELISA, WB
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survivin polyclonal antibody  (Elabscience)
N/A
This gene is a member of the inhibitor of apoptosis IAP gene family which encode negative regulatory proteins that prevent apoptotic cell death IAP family members usually contain multiple baculovirus IAP repeat BIR domains but
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anti ruvbl1 rvb1 magnetic beads immunoprecipitation ip kit  (Sino Biological)
N/A
Produced in rabbits immunized with purified recombinant Human RUVBL1 RVB1 rh RUVBL1 RVB1 Catalog 14074 H07B Q9Y265 1 Met1 Lys456 RUVBL1 RVB1 specific IgG was purified by Human RUVBL1 RVB1 affinity chromatography
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mouse parva antibody - n-terminal region (oaab14228)  (Aviva Systems)
N/A
Mouse Parva antibody - N-terminal region; Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Image Search Results


a, RV-B14 replicon or firefly luciferase mRNA control luciferase expression in wild-type (WT; H1-HeLa +CDHR3 ), SETD3 knockout (SETD3 KO ), and SETD3 stably complemented (SETD3 KO + SETD3 cDNA) cell lines. Top panel is a schematic for RV-B14 replicon construct, in which Firefly luciferase reporter gene (FLuc) was inserted to replace the majority of the structural proteins b, Schematic for enterovirus infection cycle and inhibitors that block specific stages during viral infection are indicated. Synchronized luciferase-expressing CV-B3 infection of SETD3 KO cells and WT cells treated with either 125 μM cycloheximide (CHX) or 2 mM guanidine hydrochloride (GuHCl) over a time course (right panel). c, Confocal imaging of SETD3 KO cells and SETD3 KO cells that stably expressed GFP, SETD3 fused with GFP at the N-terminal (GFP-SETD3), GFP-SETD3 fused with a nuclear localization signal (GFP-SETD3-NLS), or GFP-SETD3 fused with a nuclear export signal (GFP-SETD3-NES). A representative set of two independent experiments is shown here. Scale bars, 7 μm. d, qRT-PCR quantification of viral RNA levels in RV-C15 or EV-D68 MO infected cell lines. All of the complemented constructs were expressed under a minimal expression promoter. For panel a , b , and d, datasets represent the mean with SEM of n = 3 independent biological replicates. P -values were determined by either two-way ANOVA ( a and b ) or ordinary one-way ANOVA ( d ) with Holm-Sidak correction on log-transformed data. For panel b , at 4 hpi and beyond, all differences observed between untreated WT vs. all other conditions are statistically significant ( P < 0.0001). ns = non-significant. * = P < 0.05. ** = P < 0.0001.

Journal: Nature microbiology

Article Title: Enterovirus Pathogenesis Requires the Host Methyltransferase SETD3

doi: 10.1038/s41564-019-0551-1

Figure Lengend Snippet: a, RV-B14 replicon or firefly luciferase mRNA control luciferase expression in wild-type (WT; H1-HeLa +CDHR3 ), SETD3 knockout (SETD3 KO ), and SETD3 stably complemented (SETD3 KO + SETD3 cDNA) cell lines. Top panel is a schematic for RV-B14 replicon construct, in which Firefly luciferase reporter gene (FLuc) was inserted to replace the majority of the structural proteins b, Schematic for enterovirus infection cycle and inhibitors that block specific stages during viral infection are indicated. Synchronized luciferase-expressing CV-B3 infection of SETD3 KO cells and WT cells treated with either 125 μM cycloheximide (CHX) or 2 mM guanidine hydrochloride (GuHCl) over a time course (right panel). c, Confocal imaging of SETD3 KO cells and SETD3 KO cells that stably expressed GFP, SETD3 fused with GFP at the N-terminal (GFP-SETD3), GFP-SETD3 fused with a nuclear localization signal (GFP-SETD3-NLS), or GFP-SETD3 fused with a nuclear export signal (GFP-SETD3-NES). A representative set of two independent experiments is shown here. Scale bars, 7 μm. d, qRT-PCR quantification of viral RNA levels in RV-C15 or EV-D68 MO infected cell lines. All of the complemented constructs were expressed under a minimal expression promoter. For panel a , b , and d, datasets represent the mean with SEM of n = 3 independent biological replicates. P -values were determined by either two-way ANOVA ( a and b ) or ordinary one-way ANOVA ( d ) with Holm-Sidak correction on log-transformed data. For panel b , at 4 hpi and beyond, all differences observed between untreated WT vs. all other conditions are statistically significant ( P < 0.0001). ns = non-significant. * = P < 0.05. ** = P < 0.0001.

Article Snippet: RV-B14 replicon RNA was first linearized by SmaI (New England Biolabs) restriction digestion and then precipitated by 0.1 volume 3M sodium acetate, 0.05 volume 0.5M EDTA, and 2.5 volume 100% ethanol.

Techniques: Luciferase, Expressing, Knock-Out, Stable Transfection, Construct, Infection, Blocking Assay, Imaging, Quantitative RT-PCR, Transformation Assay

a, qRT-PCR quantification of viral RNA levels of RV-A2, RV-B14 and multiple EV-D68 strains infected human primary normal bronchial epithelial (NHBE) cells and SETD3 KO NHBE cells. Datasets represent the mean with SEM of n = 3 independent biological replicates. b, Survival curve for wild-type ( Setd3 +/+ ), heterozygous ( Setd3 +/− ), and homozygous SETD3 knockout ( Setd3 −/− ) mice injected intracerebrally with coxsackievirus A10 (CV-A10) at 150,000 infectious particles/mouse. c , Survival curve for mice injected intracerebrally with enterovirus A71 (EV-A71) at 10,000 infectious particles/mouse. For panels b and c , the number of survivors over the total number for each group of mice is indicated in parenthesis. d, qRT-PCR quantification of viral RNA levels of CV-A10 present in the hind-limb skeletal muscle at 6 dpi of indicated mice. Data represents the mean with SEM of n = 3 animals for each group of mice. e, qRT-PCR quantification of viral RNA levels of EV-A71 present in the hind-limb skeletal muscle at 4 dpi of indicated mice. Data represents the mean with SEM of n = 3 ( Setd3 +/+ ) or n = 5 ( Setd3 +/− ) mice. f, qRT-PCR quantification of viral RNA levels of enterovirus D68 (EV-D68) at 6 dpi in mice injected intramuscularly with 100,000 infectious particles/mouse at 6 dpi. Data represents the mean with SEM of n = 4 ( Setd3 +/+ ), n = 8 ( Setd3 +/− ) or n = 5 ( Setd3 −/− ) mice. For panel a , d , and f , P -values were determined by either two-way ANOVA ( a ), ordinary one-way ANOVA ( d and f ) with Holm-Sidak correction on log-transformed data. For panel b and c , Two-tailed P -values for survival of Setd3 −/− mice versus wild type were determined by log-rank (Mantel-Cox) test. For panel e , P -values were determined by two-tailed unpaired t-test on log-transformed data. ns = non-significant. * = P < 0.05. ** = P < 0.0001.

Journal: Nature microbiology

Article Title: Enterovirus Pathogenesis Requires the Host Methyltransferase SETD3

doi: 10.1038/s41564-019-0551-1

Figure Lengend Snippet: a, qRT-PCR quantification of viral RNA levels of RV-A2, RV-B14 and multiple EV-D68 strains infected human primary normal bronchial epithelial (NHBE) cells and SETD3 KO NHBE cells. Datasets represent the mean with SEM of n = 3 independent biological replicates. b, Survival curve for wild-type ( Setd3 +/+ ), heterozygous ( Setd3 +/− ), and homozygous SETD3 knockout ( Setd3 −/− ) mice injected intracerebrally with coxsackievirus A10 (CV-A10) at 150,000 infectious particles/mouse. c , Survival curve for mice injected intracerebrally with enterovirus A71 (EV-A71) at 10,000 infectious particles/mouse. For panels b and c , the number of survivors over the total number for each group of mice is indicated in parenthesis. d, qRT-PCR quantification of viral RNA levels of CV-A10 present in the hind-limb skeletal muscle at 6 dpi of indicated mice. Data represents the mean with SEM of n = 3 animals for each group of mice. e, qRT-PCR quantification of viral RNA levels of EV-A71 present in the hind-limb skeletal muscle at 4 dpi of indicated mice. Data represents the mean with SEM of n = 3 ( Setd3 +/+ ) or n = 5 ( Setd3 +/− ) mice. f, qRT-PCR quantification of viral RNA levels of enterovirus D68 (EV-D68) at 6 dpi in mice injected intramuscularly with 100,000 infectious particles/mouse at 6 dpi. Data represents the mean with SEM of n = 4 ( Setd3 +/+ ), n = 8 ( Setd3 +/− ) or n = 5 ( Setd3 −/− ) mice. For panel a , d , and f , P -values were determined by either two-way ANOVA ( a ), ordinary one-way ANOVA ( d and f ) with Holm-Sidak correction on log-transformed data. For panel b and c , Two-tailed P -values for survival of Setd3 −/− mice versus wild type were determined by log-rank (Mantel-Cox) test. For panel e , P -values were determined by two-tailed unpaired t-test on log-transformed data. ns = non-significant. * = P < 0.05. ** = P < 0.0001.

Article Snippet: RV-B14 replicon RNA was first linearized by SmaI (New England Biolabs) restriction digestion and then precipitated by 0.1 volume 3M sodium acetate, 0.05 volume 0.5M EDTA, and 2.5 volume 100% ethanol.

Techniques: Quantitative RT-PCR, Infection, Knock-Out, Injection, Transformation Assay, Two Tailed Test